Vitamin C Lab

Finding harvest-times containing highest amount of vitamin C sateIntroductionTo understand this lab, the knowledge of what vitamins be should be explained commencement ceremony. ?Vitamins argon organic (carbon containing) molecules that mainly share as catalysts for reactions deep down the body? (hypertext transfer communications protocol://www.howstuffworks.com/vitamin-c.htm). Vitamins are organic substances, which mean that they are do by plants or animals. Vitamin C, besides known as ascorbic superman, is a soluble vitamin. It is necessary for normal growth and development. The body somewhat what relies on vitamin C to form collagen in bones, cartilage, muscle, and blood vessels and support in the absorption of iron. ?Dietary saturnineces of vitamin C include fruits and ve captivateables, oddly citrus tree fruits? (http://www.nlm.nih.gov/Medline plus/druginfo/natural/patient-vitaminc.html). PurposeTo determine the vitamin C electrical depicted object of various fr uits and to find turn out which fruit has the highest vitamin c field. HypothesisIt was hypothesized that slaked lime tree allow score the highest vitamin C subject matter overdue to it world berth of the citric group and its low acid levels. To attention deficit hyperactivity disorder on, lime apprehensions really sour and this could be due to that it has a low acid level, which relates to the vitamin C content. It was also hypothesized that orange will name the second intimately vitamin c content beca practice session it is also part of the citric group. tomato will ca-ca the ternion most vitamin c content because it has a sweet taste to it, which can contribute to it possibly having a lower vitamin C content. It is also not part of the citric group. It was also hypothesized that mango tree will have the forth most vitamin C content due to that it is very sweet, and this could possibly indicate how more than than vitamin C content in that respect is. Lastly, apple will have the fifth most vitamin C co! ntent because it might be spicy in discipline vitamins. Procedure1. apply a pipette to exile 0.5 ml of 0.200 mg/ml vitamin C outcome to the first well of a 24-well plate. Repeat for the 0.400, 0.600, 0.800, and 1.00 mg/ml solution to the wells. 2. Add triad drops of starch solution indicator to distributively of these wells. 3. Place the plate on top of the white wallpaper to better observe. Use a thin pipette to add the atomic tote up 53 solution drop by drop to the 0.200 mg/l vitamin C solution. Count the drops! When the first solution turns to a permanent dark colour, the titration has been completed. 4. Produce a graph illustrating phantasma of iodine drops vs. Vitamin C concentration. Draw a line of stovepipe fit. 5. lift out all(prenominal) fruit and blend them individually to catch a water solution. 6. Repeat steps 1-3 with distributively of the fruits. Be legitimate to rinse the pipette amid samples. Record the issuance of drops in Data Table #1 then u se the graph to determine the specific vitamin C concentration for each fruit. 7. gaudy up (rinse the plates out in the sink immediately). veridical: linden treeorange treeAppleMangoTomatoThin-stem pipetteGraduated pipetteMicro-stirring rod24-Well plateVitamin C concentrations (0.200,0.400,0.600,0.800,1 mg/ml)Starch indicator iodine SolutionObservationsTable 1: Chart demonstrate the amount of iodine drops taken to titrate different fruits. This info was compared with the stock solution. commonplace Solution: Vitamin CFruitsVitamin C (mg/ml) Iodine dropsFruit Iodine Drops0.200 2Orange 230.400 3Apple 20.600 13Mango 90.800 17Tomato 71.00 22 Lime ! 28By observing the table above, a toilet of information is presumption about the vitamin C content of each fruits. The difference of vitamin C in each fruit is riddle of substantial as there are great differences between some fruits. separate fruits have similar vitamin C content as to former(a)s. ResultsFigure 1: A graph showing how much vitamin C each fruit has. DiscussionSome fruits may have higher(prenominal) vitamin C levels than other fruits due to the natural vitamins that the fruits contain.

Some fruits may be full with vitamin C but lack other vitamins and vice versa. The fruit itself can contain vitamin C and have a mound of it or elflike. By conducting this lab, we erect out which fruits had a lot of vitamin C comparing to fruits that had very little vitamin C. It basically depends on the type of fruit. There may have been a few sources of error:- Contamination of juices- Contaminated well-plate- inconclusive measurement of solutions- Incorrect measurement of iodine drops poured- Using fruits that are not ripe. Improvements: sensation improvement that can be do to this lab is apply ripe fruits as they contain more vitamin C, and this contributes to better results. ConclusionIn overall, the speculation stated was incorrect. Everything stated in the dead reckoning was correct only that mango had the third most vitamin C content quite of fourth most vitamin C content. Also, we found out that love apple had the fourth most vitamin C content, which meant that mango had more vitamin C than tomato. All other rankings in the hypothesis were cor rect. AppendicesFinding percent of vitamin C each fru! it contains with recommended unremarkable vitamin C intake. Lime:1.3 mg ascorbic acid (250 mL)/60mg * vitamin C= 542 %Orange: 1.02 mg ascorbic acid (250 mL)/60mg * one hundred= 425 %Mango:0.5 mg ascorbic acid (250 mL)/60mg *century= 208 %Tomato:0.44 mg ascorbic acid (250 mL)/60mg *100= 183 %Apple:0.18 mg ascorbic acid (250 mL)/60mg *100= 75 %ReferencesGordon, D. J. (n.d.). HowStuffWorks How Vitamin C Works. Retrieved June 11, 2009, from http://www.howstuffworks.com/vitamin-c.htmMedlinePlus Herbs and Supplements: Vitamin C (ascorbic acid) . (n.d.). Retrieved June 11, 2009, from http://www.nlm.nih.gov/medlineplus/druginfo/natural/patient-vitaminc.htmlVitamin C and Citrus Juices. (n.d.). Retrieved June 11, 2009, from http://www.ultimatecitrus.com/vitaminc.html If you exigency to get a full essay, order it on our website: OrderCustomPaper.com
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